Single organelle function and organization as estimated from Arabidopsis mitochondrial proteomics
نویسندگان
چکیده
منابع مشابه
Organelle proteomics.
This unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with organelle preparation, protein solubilization, and proteomics techniques. A specific commentary for organelle proteomics is provided. Specific protocols for the isolation of nuclei from various sources (cell cultures, ...
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mitochondria play key roles in many cell functions including energy production, fatty acid metabolism, pyrimidine biosynthesis, calcium homeostasis, and aging. they also regulate crucial signaling cascades such as apoptosis and oxidative stress. the proteome is often used to investigate the functional correlations on protein levels. based upon the human, genome there is estimated 2000 to 2500 a...
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RATIONALE Mitochondria constitute 30% of myocardial mass. Mitochondrial fusion and fission appear essential for health of most tissues. Mitochondrial fission occurs in neonatal cardiomycyte and is implicated in cardiomyocyte death. Mitochondrial fusion has not been observed in postmitotic myocytes of adult hearts, and its occurrence and function in this context are controversial. OBJECTIVE De...
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In biology, localisation is function: knowledge of the localisation of proteins is of paramount importance to assess and study their function. This supports the need for reliable protein sub-cellular localisation assignment. Concomitant with recent technological advances in organelle proteomics, there is a requirement for more rigorous experimental and analysis design planning and description. ...
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Chromatin proteins mediate replication, regulate expression, and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined. This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle, it defies classical organellar proteomics and canno...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 2019
ISSN: 0960-7412,1365-313X
DOI: 10.1111/tpj.14534